Generator

Part:BBa_K1709001:Design

Designed by: Astrid Deryckere   Group: iGEM15_KU_Leuven   (2015-09-08)


LuxI-His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Biobrick BBa_C0061 still contained a Barcode sequence which was deleted. Also, a Ribosome Binding Site was added. For our own use, 5 bp were exchanged to create unique restriction sites and to optimize DNA folding. gBlocks were used to partly design the plasmid and the ribosome binding site sequence was based purely on the Anderson library and thus, there is a mistake in the composite part sequence since the scar is only partly present.

Source

Sequence is originating from V. fischeri. Here, the sequence from BBa_C0061 was used and optimized.

References